| Model |
Vibrio 01 |
| Production Country |
China |
| Weight (kg) |
1 |
| Color |
White |
- This is for the development and testing of a novel, rapid, colorimetric immunodiagnostic kit, Cholera SMART, for direct detection of the presence of Vibrio cholerae O1 in clinical specimens.
- Unlike conventional culture methods requiring several days to complete, the Cholera SMART kit can be used directly in the field by untrained or minimally skilled personnel to detect V. cholerae O1 in less than 15 min, without cumbersome laboratory equipment.
- A total of 120 clinical and environmental bacterial strains, including both O1 and non-O1 serotypes of V. cholerae isolated from samples collected from a variety of geographical regions, were tested, and positive reactions were observed only with V. cholerae O1.
- Also, results of a field trial, employing Cholera SMART, showed 100% specificity and 96% sensitivity compared with conventional culture methods.
- Another field trial showed that Cholera SMART was 100% in agreement with a recently described coagglutination test when 108 stool specimens were tested.
Key Features
The Cholera SMART kit consists of a reaction vial and a SMART device.
The reaction vial contains a lyophilized monoclonal antibody.
The SMART device comprises two compartments. The upper compartment contains a membrane bounded by an area large enough to hold the swab used to transfer the test specimen from the reaction vial.
The lower compartment contains two immunoglobulincoated spots (a test spot coated with anti-V. cholerae 01 polyclonal antibody (1) and a negative control spot coated with a normal rabbit immunoglobulin).
The polyclonal anti-V. cholerae 01 antisera were produced in rabbits immunized with 109 CFU.
The specificity and antibody activity in each preparation of anti-V. cholerae 01 serum were tested by both slide agglutination (1) and coagglutination (4).
The kit contains reconstitution buffer (0.01 M phosphate-buffered saline containing 3% Tween 20, pH 7.3), extraction buffer (0.05 M Tris, 0.05 M EDTA, 0.1 M NaCl, pH 8.2), and a specimen-filtering device consisting of a squeezable 2-ml plastic tube with a snap-on filter (1-,um pore size) that allows bacterial cells to pass through while retaining any particulates that could interfere with the reaction.
In addition, swabs (100% Dacron) for reagent transfer or sampling are also provided in the kit.
What's In the Box
20 sets of Cholera test Kit Vibrio 01
